This presentation will focus on the development of a highly efficient callus-induced regeneration system for Queen’s crapemyrtle (L. speciosa) using plant growth regulators for callus initiation, differentiation, shoot proliferation, and rooting. The protocol’s effectiveness in producing true-to-type plants will also be confirmed using inter-simple sequence repeats (ISSR) markers. Located in Hall 2 near the TNLA PAC Lounge.
Executive Summary:
The presentation will cover the following topics:
Development of a regeneration protocol via callus-induced organogenesis from leaf pieces of Queen’s crapemyrtle.
Optimization of plant growth regulators for callus initiation, differentiation, shoot proliferation, and rooting.
Assessment of genetic fidelity using ISSR markers to confirm the production of true-to-type plants.
Potential applications of the regeneration system in genetic engineering to improve plant resistance or alter desired horticultural traits.
Takeaway Bullet Points:
Learn about the development of a highly efficient callus-induced regeneration system for Queen’s crapemyrtle.
Understand the optimization of plant growth regulators for callus initiation, differentiation, shoot proliferation, and rooting.
Gain knowledge about the assessment of genetic fidelity using ISSR markers to confirm true-to-type plant production.
Explore the potential applications of the regeneration system in genetic engineering to improve plant resistance or alter desired horticultural traits.
Gain hands-on experience with the protocol through a demonstration using plant materials in callus initiation, differentiation, shoot proliferation, and rooting stages, using a dissecting microscope.